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IDDRC Core C: Biological and Molecular Analysis Core (BMAC) Service Request

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Today's Date

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M-D-Y

Project Title

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Please specify the project title and PI name that were not found on the drop-down list

Project Title

PI Name

Grant Award Number

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Project ID #

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Select Core PI

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Pam Lein- Neuroimaging subcore

Judy Van de Water- Immunology subcore

Janine LaSalle - Genomics subcore

Immunology Service Request

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Consultation in model selection and experimental design.

Training of users on available equipment.

Training and supervision in conducting assays of interest where applicable.

Battery of assays conducted by core staff: Western Blot, ELISA, Luminex and Flow Cytometry.,

Immunohistochemical staining of tissue sections by core staff.

Available set of analysis conducted by core staff.

Other: please explain.

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Neuroimaging Request

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Primary culture of central and peripheral neurons derived from rodent models, including transgenic mice and rats.

Immunocytochemical analyses of neural cell cultures.

Biolistic and Diolistic labeling of cells in dissociated neural cell cultures or brain slice cultures.

Methods for expressing cDNA in cultured neurons.

Morphometric analyses.

Confocal microscopy.

High content imaging.

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Genomics Request

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DNA methylation analysis by pyrosequencing

Genomic and epigenomic sequencing consultation for experimental design (e.g. RNA-seq, miRNA-seq, whole genome bisulfite sequencing, ChIP-seq, chromatin capture conformation, microbiome 16S, etc.)

Fluorescence in situ hybridization

Molecular genetic methods (qRT-PCR, Western blot, Fluidigm, genotyping, etc.)

Other: please explain

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Other: please explain

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Your Project Name

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Brief Project Description:

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Project End Date Request

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M-D-Y

Project Start Date Request

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M-D-Y

PI Last Name

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PI First Name

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PI Email Address

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PI Phone Number

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Study Contact Last Name

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Study Contact First Name

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Study Contact Email

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Study Contact Phone Number

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